cd47 proteintech Search Results


95
Proteintech cd47 antibody
( A ) Distribution of the amount of clusters (indicative of EVs) per size range (nm, depicting the diameter of the clusters) detected using the different staining panels (black circle, Integrin-β1+WGA; blue square, Integrin-β1+GFRP78; red triangle, <t>CD47+GRP78).</t> ( B ) Representative images of individual EVs that were enriched in either one (left, right) or two (middle) of the indicated markers: WGA (green), integrin-β1 (magenta), GRP78 (cyan), CD47 (yellow). Each horizontal row represents one of the three staining panels. White scale bars indicate 200 nm. ( C ) The percentage of EVs (of all clusters detected in each staining panel as in A) that are single or double positive for indicated markers. Bars are colored to indicate markers: light grey, WGA; blue horizontal stripes, integrin-β1; dark grey, GRP78; red vertical stripes, CD47. ( D ) Graphical summary of the detected PCa EV subpopulations and in which cell types these markers were traced (see ). Colors indicating the proteins are similar as in C. A and C show mean±SEM of 3 fields of view. WGA, Wheat Germ Agglutinin
Cd47 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Distribution of the amount of clusters (indicative of EVs) per size range (nm, depicting the diameter of the clusters) detected using the different staining panels (black circle, Integrin-β1+WGA; blue square, Integrin-β1+GFRP78; red triangle, CD47+GRP78). ( B ) Representative images of individual EVs that were enriched in either one (left, right) or two (middle) of the indicated markers: WGA (green), integrin-β1 (magenta), GRP78 (cyan), CD47 (yellow). Each horizontal row represents one of the three staining panels. White scale bars indicate 200 nm. ( C ) The percentage of EVs (of all clusters detected in each staining panel as in A) that are single or double positive for indicated markers. Bars are colored to indicate markers: light grey, WGA; blue horizontal stripes, integrin-β1; dark grey, GRP78; red vertical stripes, CD47. ( D ) Graphical summary of the detected PCa EV subpopulations and in which cell types these markers were traced (see ). Colors indicating the proteins are similar as in C. A and C show mean±SEM of 3 fields of view. WGA, Wheat Germ Agglutinin

Journal: bioRxiv

Article Title: EV trace : tracing extracellular vesicles-associated proteins in recipient cells using stable isotope labeling

doi: 10.1101/2025.11.23.690082

Figure Lengend Snippet: ( A ) Distribution of the amount of clusters (indicative of EVs) per size range (nm, depicting the diameter of the clusters) detected using the different staining panels (black circle, Integrin-β1+WGA; blue square, Integrin-β1+GFRP78; red triangle, CD47+GRP78). ( B ) Representative images of individual EVs that were enriched in either one (left, right) or two (middle) of the indicated markers: WGA (green), integrin-β1 (magenta), GRP78 (cyan), CD47 (yellow). Each horizontal row represents one of the three staining panels. White scale bars indicate 200 nm. ( C ) The percentage of EVs (of all clusters detected in each staining panel as in A) that are single or double positive for indicated markers. Bars are colored to indicate markers: light grey, WGA; blue horizontal stripes, integrin-β1; dark grey, GRP78; red vertical stripes, CD47. ( D ) Graphical summary of the detected PCa EV subpopulations and in which cell types these markers were traced (see ). Colors indicating the proteins are similar as in C. A and C show mean±SEM of 3 fields of view. WGA, Wheat Germ Agglutinin

Article Snippet: Unconjugated integrin-β1 antibody (clone P5D2, Santa Cruz Biotechnology, Dallas, TX, USA), CD47 antibody (clone B6.H12, InVivo MAb), or mouse IgG1 isotype control (clone P3.6.2.8.1, eBioscience, Invitrogen) were labeled with mouse IgG1 targeting nanobodies (VHH-AlexaFluor568, VHH-AlexaFluor647, Proteintech, Rosemont, IL, USA).

Techniques: Staining